PTM Viewer PTM Viewer

AT2G14750.1

Arabidopsis thaliana [ath]

APS kinase

11 PTM sites : 3 PTM types

PLAZA: AT2G14750
Gene Family: HOM05D001721
Other Names: AKN1,APS KINASE 1,APK1,ADENOSINE-5'-PHOSPHOSULFATE (APS) KINASE 1,ATAKN1; APK

Link out to other resources with this protein ID : TAIR   |   PeptideAtlas   |   ARAPORT   |   PhosPhAt

For each protein all PTMs are highlighted by default in the respective protein sequence (right-hand side). One can adjust a selection of PTMs in the PTM table on the left-hand side. In addition, functional protein domains and sites can be underlined if desired.

In the PTM table per PTM the PTM position and type is indicated, as well as the plain peptide sequence that was identified by mass spectrometry. The respective proteomics study is indicated by a number, providing a link to consult the experimental details. Additional PTM meta-data includes various confidence measures such as peptide score provided by search algorithms, posterior error probability (PEP), precursor mass deviation (in ppm) and modification site probability. The available confidence meta-data can be consulted in the extended PTM table by clicking SHOW CONFIDENCE. However, in the default PTM table, a color-coding of confidence is provided with green indicating high confidence, olive medium confidence, grey low confidence, and no color an unassigned confidence. More details regarding this confidence assignment can be consulted in the tutorial or the Plant PTM Viewer manuscript.

Besides confidence measures, log2 fold changes between two conditions with significance values (P- or Q-values) are shown if provided in the respective publication. Log2 fold changes are colored in heatmap-like gradient (green = induced, red = repressed) and significant values are highlighted in green. To determine significance, we employed the threshold used in the respective publication. For more details on the quantitative measurements we refer to the experimental details and respective publication, as methodologies can differ.

On the bottom of the page one can send the whole protein or a part of the protein (i.e. a functional domain) to PTM Blast. This will display aligned protein sequences that potentially report aligned PTMs.

PTMs



PTM Type

Mod AA

Pos

Peptide

Exp ID

Conf
ph S 23 GIFDSNSMSNSR114
ph S 25 GIFDSNSMSNSR114
ph S 40 ACVSMDGSQTLSHNK114
ph S 54 NGSIPEVK43
ph T 74 QGPLSTVGNSTNIK114
ph S 78 QGPLSTVGNSTNIK114
sno C 86 WHECSVEK169
ox C 132 LCYILDGDNVR138b
ph S 149 DLSFKAEDR114
ox C 245 GFTGIDDPYEPPLNCEISLGR138b
sno C 245 GFTGIDDPYEPPLNCEISLGR90b

Sequence

Length: 276

MIAAGAKSLLGLSMASPKGIFDSNSMSNSRSVVVVRACVSMDGSQTLSHNKNGSIPEVKSINGHTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA

ID PTM Type Color
ph Phosphorylation X
sno S-nitrosylation X
ox Reversible Cysteine Oxidation X
Multiple types X

Domains & Sites

Clear highlighted range 
Molecule Processing
Show Type From To
Transit Peptide 1 38
Sites
Show Type Position
Metal Ion-binding Site 150
Site 182
Active Site 108
Active Site 138
Active Site 141
Active Site 155
Active Site 158
Active Site 231
Active Site 181

BLAST


Perform a BLAST search for this sequence, or a part of this sequence (minimum 50 characters)
A downloadable tutorial can be found here